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A mixed self-assembled monolayers(SAMs)of thioctic acid(T-COOH)and thioctic acid amide(T-NH2)were used to immobilize tyrosinase for fabricating biosensor.The results showed that the mixed SAMs prepared from solution at the ratio of 1:4provided an excellent microenvironment for enzymatic reaction between tyrosinase and substrate.The biosensor exhibited a fast response and high sensitivity for sensing substrate.