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用重组人白细胞介素-2(IL-2)含信号肽cDNA的缺陷型逆转录病毒,将IL-2基因转导入小鼠H_(22)肝癌细胞株,经PCR和RT-PCR检测证实了转导后的细胞DNA内确有NeoR和IL-2基因的存在的表达。分别用光镜和电镜观察了经IL-2基因转录的H_(22)细胞体外形态,MTT法检测细胞体外增殖能力以及皮下接种H_(22)-IL-2细胞后观察肿瘤生长能力。结果表明经IL-2基因转导的鼠肝癌细胞体外增殖能力没有明显变化,但体内致瘤性却显著下降,丝裂霉素处理后作为瘤苗接种能抑制其后野生型H_(22)细胞的再次攻击。本研究为制备新型瘤苗打下了基础。
The recombinant human interleukin-2 (IL-2)-deficient retrovirus containing the signal peptide cDNA was used to transduce the IL-2 gene into the mouse H_22 hepatoma cell line and confirmed by PCR and RT-PCR. The presence of NeoR and IL-2 genes was indeed expressed in the transduced cell DNA. The in vitro morphology of H22 cells transfected with IL-2 gene was observed by light and electron microscopy. The ability of cell proliferation in vitro was detected by MTT assay and the ability of tumor growth was observed after subcutaneous inoculation of H22-IL-2 cells. The results showed that the proliferation of murine hepatoma cells transduced with IL-2 gene had no significant changes in vitro, but the tumorigenicity in vivo was significantly decreased. After inoculation with mitomycin C, the tumor cells were inhibited by wild-type H_(22) cells. Attack again. This study laid the foundation for the preparation of new tumor vaccines.