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目的观察机动车尾气型空气污染对大鼠支气管肺泡灌洗液的影响,探讨交通干道旁生物暴露形式可能对气道炎症产生的影响。方法健康SD大鼠60只,雌雄各半,随机分为6组,暴露组大鼠置于机动车尾气污染严重的交通主干道旁,对照组置于实验室的正常环境下。分别于14、30、90d对大鼠的支气管-肺泡灌洗液(BALF)中白细胞总数(WBC)及一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)、白介素-8(IL-8)的含量进行测定。结果30d暴露组大鼠BALF中NO含量[(9.75±4.78)μmol/L]显著高于对照组[(4.40±1.45)μmol/L],而BALF中TNF-α、IL-8含量在同一观察期内两组之间差异无统计学意义(P>0.05)。结论现场动物暴露实验可观察到早期气道炎症,暴露30d为指标改变的敏感期。
Objective To observe the effect of motor vehicle exhaust air pollution on bronchoalveolar lavage fluid (BALF) in rats and to explore the possible influence of biological exposure forms on airway inflammation. Methods Sixty healthy SD rats were randomly divided into 6 groups. The rats in the exposed group were placed beside the traffic trunk road with serious vehicle exhaust pollution, and the control group was placed in the normal environment of the laboratory. The total number of white blood cells (WBC), nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-8 (IL) in bronchoalveolar lavage fluid (BALF) -8) content of the determination. Results The level of NO in BALF of rats exposed to 30d was significantly higher than that of control ([(4.75 ± 4.78) μmol / L vs [4.40 ± 1.45] μmol / L] There was no significant difference between the two groups during the period (P> 0.05). Conclusion The early airway inflammation can be observed in the field animal exposure experiment, and the 30d exposure is the sensitive period of the index change.