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采用紫外吸收光谱法、循环伏安法和微分脉冲伏安法研究了一种水杨酰胺多吡啶铜配合物[Cu(phen)(sa)](phen=1,10-邻菲罗啉,sa=水杨酰胺)与DNA的相互作用。紫外光谱法显示DNA的加入能引起配合物特征吸收峰的减色效应,表明了二者之间的相互作用。循环伏安实验表明配合物在玻碳电极上呈现一对准可逆的氧化还原峰;加入DNA后,配合物的峰电流减小,峰电位正移,表明二者可能通过嵌插方式发生作用。微分脉冲伏安法进一步表明[Cu(phen)(sa)]作为电化学探针,能在1.3×10-5～6.7×10-5moL/L DNA浓度范围内对DNA进行定量检测。将该铜配合物作为杂交指示剂应用于DNA传感器中对花椰菜花叶病毒的35S启动子基因(CaMV35S)相关DNA片段进行检测,结果显示该传感器对互补序列和非互补序列具有很好的识别能力。
A novel polypyridine copper salicylate complex [Cu (phen) (sa)] (phen = 1,10-phenanthroline, sa = Salicylamide) and DNA interaction. UV spectroscopy showed that the addition of DNA can cause the subtractive effect of the characteristic absorption peak of the complex, indicating the interaction between the two. Cyclic voltammetry experiments showed that the complex exhibited a redox peak at the glassy carbon electrode. After the addition of DNA, the peak current of the complex decreased and the peak potential shifted positively, indicating that the two compounds may act by intercalation. Differential pulse voltammetry further demonstrated that [Cu (phen) (sa)] can be used as an electrochemical probe to quantitatively detect DNA in the concentration range of 1.3 × 10-5 ~ 6.7 × 10-5moL / L DNA. The copper complex was used as a hybridization indicator in the DNA sensor to detect cauliflower mosaic virus 35S promoter gene (CaMV35S) DNA fragment was detected, the results show that the sensor of complementary and non-complementary sequences have good recognition ability .