Numerical quadrature is an important ingredient of Galerkin meshless methods. A new numerical quadrature technique, partition of unity quadrature (PUQ),for Galerkin meshless methods was presented. The technique is based on finite covering and partition of unity. There is no need to decompose the physical domain into small cell. It possesses remarkable integration accuracy. Using Element-free Galerkin methods as example, Galerkin meshless methods based on PUQ were studied in detail. Meshing is always not required in the procedure of constitution of approximate function or numerical quadrature, so Galerkin meshless methods based on PUQ are "truly"meshless methods.