论文部分内容阅读
目的探讨网织红细胞染色的改良方法。方法选取增生性贫血患者外周血,分别用改良法和常规法在不同时间,不同温度下染色,然后推片计数。结果改良法染色效果更佳,杂质和细胞碎片明显减少,时间对改良法染色结果影响较小。结论在保证同等质量前提下,改良法染色网织红细胞,减少了染色液配制的繁琐,延长了其保存时间。改良法染色网织红细胞,细胞碎片、杂质等均少于常规法,同时降低了染色对时间的要求。
Objective To explore the improved method of reticulocyte staining. Methods Peripheral blood of patients with hyperplastic anemia were selected and stained by modified method and conventional method at different time and different temperature, then counted by push-piece. Results The results of the improved method showed better staining, fewer impurities and cell debris, and the time had little effect on the results of the modified method. Conclusion Under the premise of ensuring the same quality, the improved method of staining reticulocytes, reducing the cumbersome preparation of the dyeing liquid, extending its storage time. Modified method staining reticulocytes, cell debris, impurities, etc. are less than the conventional method, while reducing the staining of the time requirements.