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研究地塞米松（Ｄｅｘ）和布洛芬（Ｉｂｕ）对脂多糖（ＬＰＳ）诱导的大鼠肺ＴＮＦα、ＩＬ-１β和ＭＩＰ-１α基因表达的影响．方法：荧光法测定肺中伊文斯蓝含量，Ｓｌｏｔｂｌｏｔ对细胞因子ｍＲＮＡ表达相对定量．结果：腹腔注射ＬＰＳ导致大鼠肺中ＴＮＦα、ＩＬ-１β和ＭＩＰ-１αｍＲＮＡ表达明显增加，与ＬＰＳ剂量有依赖关系，峰值分别在２，６，１２小时．在ＬＰＳ前１小时给药，Ｄｅｘ５０ｍｇ·ｋｇ－１和Ｉｂｕ９０ｍｇ·ｋｇ－１均明显降低肺中伊文斯蓝含量，同时ＴＮＦα、ＩＬ-１β和ＭＩＰ-１αｍＲＮＡ表达量亦明显减少．结论：ＬＰＳ诱导大鼠肺中ＴＮＦα、ＩＬ-１β和ＭＩＰ-１α基因表达，Ｄｅｘ和Ｉｂｕ通过抑制细胞因子表达而减轻肺损伤．
To investigate the effects of dexamethasone (Dex) and ibu on the expression of TNFα, IL-1β and MIP-1α in rat lung induced by lipopolysaccharide (LPS). Methods: The contents of Evans blue in lungs were measured by fluorescence spectrophotometry. Relative expression of cytokine mRNA was determined by Slotblot. Results: The expression of TNFα, IL-1β and MIP-1αmRNA in the lungs of rats were significantly increased after intraperitoneal injection of LPS, which was dependent on the dose of LPS at 2,6 and 12 hours respectively. One hour before LPS administration, Dex50mg · kg-1 and Ibu90mg · kg-1 significantly reduced the Evans blue content in the lungs, meanwhile TNFα, IL-1β and MIP-1α mRNA expression levels were also significantly reduced. CONCLUSION: LPS induces TNFα, IL-1β and MIP-1α gene expression in rat lungs. Dex and Ibu reduce lung injury by inhibiting cytokine expression.