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B 因子为补体旁路激活的重要组分,又称为 C_3激活因子前体(C_3PA),它的功能是激活 C_3(转变为 C_3激活因子后),直接活化补体系统。B 因子的测定对于了解旁路系统功能、肾小球肾炎的变化及分类、肝病、恶性肿瘤等皆有较大的价值。但因为国内无抗 B 因子血清,迟迟未见测试报告。本报告采用 Rivanol 沉淀去除优球蛋白,盐析沉淀,CM-Sephadex-C50离子交换,DEAE-A50 柱和Sephadex G-200层析从血中提取 B 因子,取得纯品后免疫绵羊,首次0.5g 加完全佐剂,经5次免疫后取得
Factor B is an important component of complement activation, also known as C_3 activator precursor (C_3PA). Its function is to activate C_3 (after conversion to C_3 activator) and activate the complement system directly. The determination of factor B is of great value in understanding the function of the bypass system, changes and classification of glomerulonephritis, liver diseases and malignant tumors. But because there is no anti-factor B serum in the country, there has been no test report yet. In this report, Rivanol precipitation to remove euglobulin, salt precipitation, CM-Sephadex-C50 ion exchange, DEAE-A50 column and Sephadex G-200 chromatography to extract B factor from blood, pure sheep after immunization, the first 0.5g Add complete adjuvant, obtained after 5 immunizations