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目的观察JAK2激酶抑制剂AG490对结肠癌HT-29细胞侵袭以及对基质金属蛋白酶-2(MMP-2)表达的影响,探讨STAT3信号转导通路在结肠癌侵袭转移调控中的作用。方法应用JAK2激酶抑制剂AG490处理结肠癌HT-29细胞,Matrigel肿瘤体外侵袭模型检测肿瘤细胞侵袭;Western blot检测STA33蛋白表达;逆转录-聚合酶链反应(RT-PCR)检测MMP2 mRNA表达。结果ACA90可抑制JAK2/STA33信号转导通路活化及结肠癌HT-29细胞侵袭,在此过程中AG490在mRNA水平抑制MMP-2表达。结论STA33信号转导通路参与结肠癌细胞侵袭调控,阻断STA33通路活化可抑制结肠癌细胞侵袭,这一作用可能是通过抑制MMP-2表达实现的。
Objective To investigate the effect of JAK2 kinase inhibitor AG490 on the invasion of human colon cancer HT-29 cells and the expression of matrix metalloproteinase-2 (MMP-2), and to explore the role of STAT3 signal transduction pathway in the invasion and metastasis of colon cancer. Methods The colon cancer HT-29 cells were treated with JAK2 kinase inhibitor AG490. The invasion of tumor cells was detected by Matrigel invasion in vitro. The expression of STA33 protein was detected by Western blot. The expression of MMP2 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results ACA90 inhibited the activation of JAK2 / STA33 signal transduction pathway and invasion of colon cancer HT-29 cells. In the process, AG490 inhibited the expression of MMP-2 at the mRNA level. Conclusion STA33 signal transduction pathway is involved in the invasion and metastasis of colon cancer cells. Blocking the activation of STA33 pathway can inhibit the invasion of colon cancer cells, which may be through inhibiting the expression of MMP-2.