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目的研究水飞蓟籽油的亚慢性毒性。方法将80只大鼠随机分为3个实验组和1个空白对照组,每组20只,雌雄各半,剂量分别为10.00 ml/kg BW、5.00 ml/kg BW和2.50 ml/kg BW,连续灌胃91 d。试验期间每周称一次体重,计算食物利用率;实验中期和末期检查大鼠血常规、生化指标;试验结束时处死动物,称量重要脏器重量,计算脏器指数,并对主要脏器进行病理组织学观察。结果实验期间,各组大鼠均未见明显中毒体征,也未见死亡。各实验组大鼠的体重和食物利用率与对照组比较差别无统计学意义。试验中期、末期血生化和血常规指标与对照组比较差别均无统计学意义。各实验组动物的脏器/体重比与对照组比较差别均无统计学意义,组织病理学未见明显异常。结论在本实验条件下,水飞蓟籽油无明显亚慢性毒性。
Objective To study the subchronic toxicity of silymarin seed oil. Methods Eighty rats were randomly divided into three experimental groups and one blank control group, with 20 mice in each group. The doses were 10.00 ml / kg BW, 5.00 ml / kg BW and 2.50 ml / kg BW respectively. Continuous oral gavage 91 d. The weight of each week during the test, calculate the food utilization rate; the middle and end of the experiment to check the blood and biochemical indicators of rats; the animals were sacrificed at the end of the experiment weighing the weight of vital organs, organ index calculation, and the main organs Histopathological observation. Results During the experiment, no obvious signs of poisoning and no death were found in all the rats in each group. There was no significant difference in body weight and food utilization between the experimental groups and the control group. There was no significant difference in the blood biochemical and hematological parameters between the middle and the end of the experiment and the control group. The organ / body weight ratio of each experimental group was no significant difference compared with the control group, histopathology no obvious abnormalities. Conclusion Silybum marianum seed oil has no obvious sub-chronic toxicity under the experimental conditions.