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目的:观察“通利大肠”对慢性阻塞性肺疾病(COPD)模型大鼠肺组织γ-谷氨酰半胱氨酸合成酶(γ-GCS)、核因子-2-相关因子-2(Nrf2) mRNA表达的影响,探讨COPD“从肠论治”效应产生的肺肠联络机制。方法:采用气管注脂多糖加熏香烟联合造模方法建立COPD大鼠模型,随机分为正常组、模型组、治肺组、治肠组及肺肠同治组。正常组、模型组灌胃生理盐水,各给药组灌胃相应中药,连续14d。RT-PCR法检测肺组织γ-GCS、Nrf2 mRNA表达水平。结果:与正常组比较,模型组大鼠肺组织γ-GCS、Nrf2 mRNA表达明显增强(P<0.01)。与模型组比较,各用药组γ-GCS、Nrf2 mRNA表达减少(P<0.01)。与治肺组比较,肺肠同治组γ-GCS、Nrf2 mRNA表达减少(P<0.01)。结论:通利大肠,或在治肺的基础上增加通利大肠,均能减少COPD大鼠肺组织γ-GCS、Nrf2mRNA的表达,这可能是COPD“从肠论治”效应产生的作用环节之一。
OBJECTIVE: To observe the effect of Tongli Stomach on the expression of γ-glutamylcysteine synthase (γ-GCS) and nuclear factor-2-related factor-2 in lung of chronic obstructive pulmonary disease (COPD) (Nrf2) mRNA expression in rats and to explore the mechanism of lung-intestine connection induced by COPD. Methods: COPD rat models were established by intratracheal injection of lipopolysaccharide and combined with cigarette smoking, and were randomly divided into normal group, model group, treatment of lung group, treatment of intestine group and treatment of lung intestine. Normal group, model group, gavage saline, each administration group gavage corresponding Chinese medicine, continuous 14d. The expression of γ-GCS and Nrf2 mRNA in lung tissue were detected by RT-PCR. Results: Compared with the normal group, the expression of γ-GCS and Nrf2 mRNA in the model group was significantly increased (P <0.01). Compared with the model group, the expression of γ-GCS and Nrf2 mRNA in each group decreased (P <0.01). Compared with the treatment group, the expression of γ-GCS and Nrf2 mRNA in the lung-intestine group decreased (P <0.01). CONCLUSION: Tongli’s large intestine, or Tongliao’s intestine, can reduce the expression of γ-GCS and Nrf2mRNA in the lung tissues of COPD rats, which may be the result of COPD One of the links.