首次提出Cu+-H2 O2 -茜素紫分析新体系并用于羟自由基的测定。本方法用Cu+催化H2 O2 反应产生·OH ;加入茜素紫显色剂使茜素紫的颜色发生变化 ,用紫外 -分光光度计测其ΔA值的变化 ,可间接测定羟自由基的产生量。通过测定条件的研究 ,得出最佳实验条件。结果表明 ,该方法稳定性好 ,操作简便 ,测定快速 ,可作为一种简便的筛选抗氧化剂的方法 For the first time, a new Cu + -H2 O2-alizarin violet analysis system was proposed and applied to the determination of hydroxyl radical. The method uses Cu + to catalyze the reaction of H2O2 to generate · OH; the alizarin violet color reagent is added to change the color of alizarin violet, and the change of ΔA value by UV-spectrophotometer can indirectly determine the amount of hydroxyl radical produced . By measuring the conditions of the study, the best experimental conditions. The results show that the method is stable, easy to operate, rapid determination can be used as a simple screening method of antioxidants