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本研究以橡胶树7-33-97悬浮细胞为材料,用核黄素进行诱导处理,并初步探索了其诱导机制。结果表明,核黄素处理橡胶树7-33-97悬浮细胞后,产生了活性氧迸发,该变化可以被钙离子通道抑制剂LaCl_3抑制;核黄素诱导橡胶树7-33-97悬浮细胞胞外基质碱性化,该变化能被蛋白激酶抑制剂K252a部分抑制,表明蛋白质磷酸化参与了该抗病过程的传导;苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)的活性被诱导而明显升高;核黄素诱导了防卫相关基因HbPR-1和HbNPR1的表达。说明核黄素能够诱导橡胶树7-33-97悬浮细胞产生抗病防卫反应,该抗病信号传导途径可能是通过水杨酸信号通路进行的。
In this study, rubber tree 7-33-97 suspension cells as a material, with riboflavin induction treatment, and initially explored its induction mechanism. The results showed that riboflavin treatment of rubbery 7-33-97 suspension cells, the generation of reactive oxygen species, the change can be calcium channel inhibitor LaCl_3 inhibition; riboflavin-induced rubber tree 7-33-97 suspension cells extracellular matrix This change was partially inhibited by K252a, a protein kinase inhibitor, suggesting that phosphorylation of proteins is involved in the resistance process. Activity of phenylalanine ammonia lyase (PAL) and peroxidase (POD) Induced and significantly increased; riboflavin induced defense-related genes HbPR-1 and HbNPR1 expression. Riboflavin can induce the rubbery 7-33-97 suspension cells produce disease-resistant defense response, the resistance signal transduction pathway may be through the salicylic acid signal pathway.