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在F344雄性大鼠腹腔注射血蓝蛋白(KLH),研究T 细胞依赖抗原初始免疫反应对中枢神经系统5 羟色胺(5 HT)代谢的影响。当免疫刺激大鼠4天后,其下丘脑匀浆5 羟色胺水平出现降低,同时应用在体脑微透析技术研究发现清醒大鼠腹腔注射血蓝蛋白4天后,其下丘脑前叶细胞外液的5 羟色胺水平增加。为了评价在免疫反应中大鼠下丘脑5 羟色胺释放效应,本文应用氯苯丙胺(PCA)选择性预先耗竭5 羟色胺释放,并测定这些动物在注射血蓝蛋白后抗体产生情况。结果显示在应用氯苯丙胺预处理的血蓝蛋白免疫动物特异抗体IgM和IgG与未用氯苯丙胺预处理的动物比较均有明显增加(P<0.01)。上述资料表明,对于T 细胞依赖抗原,其初始抗体产生的量是由位于下丘脑5 羟色胺神经终端抑制性递质释放所调节。
Cytokinin (KLH) was injected intraperitoneally into F344 male rats to study the effects of T cell-dependent antigen immunosuppression on 5 HT metabolism in central nervous system. 4 days after immunostimulation, hypothalamus homogenate serotonin levels decreased, while the application of brain microdialysis study found that conscious rats 4 days after intraperitoneal injection of hemocyanin, hypothalamic anterior lobe extracellular fluid 5 Serotonin levels increased. In order to evaluate the effect of serotonin release in rat hypothalamus during immune response, PCA was used to selectively pre-deplete serotonin release and the antibody production of these animals after injection of hemocyanin was measured. The results showed that the immunoglobulin IgM and IgG immunized with chlorpromazine pretreated with chlorpromazine significantly increased compared with those pretreated with no amphetamine (P <0.01). The above data indicate that the initial antibody production of T cell-dependent antigens is modulated by the release of inhibitory neurotransmitters located at the serotonergic terminals of the hypothalamus.