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近年我们用间接免疫酶标抗体技术,对22例外科手术切除的人体原发性肝细胞癌标本进行了有关甲胎蛋白(AFP)的组织内定位及其在光学显微镜的形态学观察研究。其结果: 一、AFP主要存在于部分肝癌细胞和癌周宿主肝细胞原浆中。阳性颗粒在细胞浆内的分布可分四型,多数弥漫分布,少数局限分布,或呈胞膜型和核周型。含AFP的肝癌细胞或宿主肝细胞在组织中多呈散在随机分布,但密度不一,个体差异较大。一般以癌组织的周边及靠血窦的癌细胞染色较强,酶标阳性颗粒也较粗大、清楚。癌周宿主肝组织亦以小叶外周或肝内静脉分支周围的细胞较为突出。
In recent years, we used indirect immuno-enzyme-labeled antibody technology to perform in- tissue localization of alpha-fetoprotein (AFP) in 22 surgically resected human primary hepatocellular carcinoma specimens and its morphological observation under optical microscope. The results: First, AFP mainly exists in some hepatocellular carcinoma cells and hepatic cancer cells in the host. The distribution of positive particles in the cytoplasm can be divided into four types, most of which are diffusely distributed, a few are limitedly distributed, or are cell membrane type and perinuclear type. AFP-containing hepatoma cells or host hepatocytes are scattered randomly throughout the tissues, but the density is not uniform and individual differences are large. In general, the cancerous tissues and the sinusoidal cancer cells stain strongly and the enzyme-labelled positive particles are also thick and clear. Pericarvular host liver tissue is also prominent in the periphery of the lobular or intrahepatic vein.