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目的 :建立高效液相色谱 (HPLC)测定鱼血和鱼肝中麻醉剂间氨基苯甲酸乙酯甲烷磺酸盐 (MS_2 2 2 )浓度的方法。方法 :样品用重蒸水匀浆 ,离心 (12 0 0 0r/min) 15min ,将上清液用 0 .45 μm过滤膜过滤后进YWG_C18柱分析 ,流动相为 0 .2mol/L磷酸缓冲液 (pH 3.1)∶甲醇 =85∶15 ,紫外检测波长 2 2 3nm。结果 :所得标准曲线的回归方程为鱼血 :Y (ng) =0 .5 2 5 8- 0 .0 894X ,相关系数γ =0 .9939,鱼肝 :Y (ng) =- 0 .10 0 +0 .110 0X ,γ=0 .996 5 ,X为峰高。平均回收率为鱼血 87.78%、鱼肝 76 .6 6 %。结论 :本法操作简便 ,重现性和回收率均较好。
OBJECTIVE: To establish a method for the determination of the concentration of ethyl aminobenzoate methanesulfonate (MS_222) among the anesthetic agents in fish blood and fish liver by high performance liquid chromatography (HPLC). Methods: The sample was homogenized with distilled water, centrifuged (12 000 r / min) for 15 min, the supernatant was filtered with 0.45 μm filter membrane and then analyzed by YWG_C18 column. The mobile phase was 0.2 mol / L phosphate buffer solution pH 3.1): methanol = 85:15, UV detection wavelength 2223nm. Results: The regression equation of the obtained standard curve was fish blood: Y (ng) = 0.5258-0.0894X, correlation coefficient γ = 0.9939, fish liver: Y (ng) = -0.100 +0 .110 0X, γ = 0 .996 5, X is the peak height. The average recovery rate was 87.78% for fish blood and 76.66% for fish liver. Conclusion: This method is simple, reproducible and good recovery.