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目的:建立大鼠血浆中麦冬多糖MDG-1的高效凝胶色谱测定方法。方法:血浆样品经30%三氯乙酸沉淀蛋白后,以0.1 M NaH_2PO_4-Na_2HPO_4缓冲液(pH 7.0)为流动相,色谱柱为Shodex Sugar ks-802凝胶柱,采用荧光检测器进行检测。结果:该法线性范围为0.25～50μg·ml~(-1)(r=0.998 9),低、中、高3个浓度的回收率为93.78%～104.6l%,日内、日间RSD低于6.74%。结论:该法准确、灵敏,操作简单,可用于血浆中MDG-1的含量测定。
Objective: To establish a high performance gel permeation chromatographic method for the determination of polysaccharide MDG-1 in rat plasma. METHODS: Plasma samples were precipitated with 30% trichloroacetic acid and the mobile phase was 0.1 M NaH2PO4-Na2HPO4 buffer (pH 7.0). The column was a Shodex Sugar ks-802 column and detected by a fluorescence detector. Results: The linear range of the method was 0.25-50 μg·ml -1 (r = 0.998 9). The recoveries of low, medium and high concentrations were 93.78% - 104.61%. 6.74%. Conclusion: The method is accurate, sensitive and easy to use. It can be used for the determination of MDG-1 in plasma.