论文部分内容阅读
本文研究了凡纳滨对虾在低溶解氧条件下酚氧化酶原系统的激活机制。结果表明:低溶氧(1.5mg/L,3.0mg/L)胁迫对凡纳滨对虾血淋巴中多巴胺含量、血细胞数量、酚氧化酶原激活系统相关酶活力、血淋巴中类α2-巨球蛋白活力影响显著(P<0.05),且各指标表现出明显的时间效应性。在实验时间内,DO为3.0mg/L处理组,总血细胞、小颗粒细胞和透明细胞数量均在12h内降低而后趋于稳定,大颗粒细胞数量在24h内降低后保持稳定,DO为1.5mg/L处理组,总血细胞数量和三类血细胞数量均在24h内降低而后趋于稳定;DO为3.0mg/L和1.5mg/L处理组凡纳滨对虾血淋巴中多巴胺含量分别在24h、12h内呈峰值变化,至12h、6h时达到最大值,在24h、12h后恢复至对照组水平并趋于稳定;血细胞中酚氧化酶原活力分别在12h、6h时显著降低,且均在24h时趋于稳定;丝氨酸蛋白酶活力在6h时均显著降低,在24h时达到最低值,而后趋于稳定,蛋白酶抑制剂活力显著降低,但类α2-巨球蛋白活力48h内呈峰值变化,与对照组比较均显著升高,48h后趋于稳定。实验期内对照组DO为5.5mg/L各指标变化不显著。
In this paper, we studied the activation mechanism of the phenolic oxidase proenzyme system of Litopenaeus vannamei under low dissolved oxygen conditions. The results showed that low dissolved oxygen (1.5mg / L, 3.0mg / L) had significant effects on the dopamine content, blood cell number, enzyme activity related to phenoloxidase system, hemolymph α2- The protein vitality had a significant effect (P <0.05), and each index showed obvious time effect. During the experimental period, the number of total blood cells, small granulosa cells and clear cells in DO treated group was reduced to 3.0mg / L within 12h and then stabilized. The number of large granulosa cells remained stable within 24h and the DO was 1.5mg / L treatment group, the total number of blood cells and the number of three types of blood cells decreased within 24 hours and then stabilized; DO 3.0mg / L and 1.5mg / L treatment group hemophthalmophthalmus dopamine levels were 24h, 12h Peaked at 12h and 6h, reached the maximum at 6h and recovered to the control group after 24h and 12h. The activity of phenoloxidase in blood cells decreased significantly at 12h and 6h The activity of serine protease decreased significantly at 6h and reached its lowest value at 24h, then stabilized. The activity of protease inhibitor decreased significantly, but the activity of α2-macroglobulin peaked within 48h. Compared with control group Compared with significantly increased, after 48h tends to be stable. During the experimental period, the control group DO was 5.5mg / L, the indexes did not change significantly.