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目的:研究药用植物巫山淫羊藿组织培养技术,为工厂化育苗提供科学根据。方法:以巫山淫羊藿的种胚为外植体,采用MS培养基,附加不同浓度2,4-D,6-BA,IBA,NAA进行正交试验。结果:诱导愈伤组织的最优培养基为:MS+2,4-D2 mg.L-1+IBA 2 mg.L-1+NAA 0.5 mg.L-1;愈伤组织分化的最优培养基为:MS+6-BA 1 mg.L-1+NAA 0.5 mg.L-1+IBA 1 mg.L-1;诱导芽的最优培养基为:MS+IBA 2 mg.L-1+6-BA 0.5 mg.L-1;芽增殖的最佳培养基为:MS+6-BA 1.0 mg.L-1+NAA 0.5 mg.L-1。结论:通过诱导愈伤组织途径和丛生芽途径,建立了巫山淫羊藿种胚外植体诱导和培养方法,达到快速繁殖的目的。
Objective: To study the tissue culture technology of medicinal plant Wushan Epimedium, providing scientific basis for plant breeding. Methods: The embryos of Epimedium wushanense were used as explants. MS medium supplemented with different concentrations of 2,4-D, 6-BA, IBA and NAA were used as explants. Results: The optimal culture medium for callus induction was: MS + 2,4-D2 mg.L-1 + IBA 2 mg.L-1 + NAA 0.5 mg.L-1; The optimal medium for inducing buds was MS + 6-BA 1 mg.L-1 + NAA 0.5 mg.L-1 + IBA 1 mg.L-1; MS + IBA 2 mg.L-1 + 6-BA 0.5 mg.L-1. The best medium for bud proliferation was MS + 6-BA 1.0 mg.L-1 + NAA 0.5 mg.L-1. Conclusion: The method of induction and culture of epimedium explants of Epimedium wuenshanense was established by inducing callus pathways and clumping bud pathways to achieve the purpose of rapid propagation.