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在已知参数条件下 ,通过电场诱导酸橙 (Cit rusaurantiumL .)叶肉原生质体和沙漠蒂甜橙(C .sinensisOsbeckcv .Shamouti)的胚性愈伤组织原生质体融合 ,融合产物经培养再生出 40棵植株。染色体检查表明所得到的植株具有 3 6条染色体 ,为四倍体植株。再生植株具有翼叶 ,叶片厚 ,表现出多倍体的特征。采用 2个 1 0 碱基随机引物鉴别再生植株的杂种特性。在 2个引物的扩增带型中 ,再生植株的随机扩增带图里出现了融合亲本的特征带。对再生植株染色体计数和RAPD分析的结果表明它们是酸橙和甜橙种间异源四倍体体细胞杂种植株。这些体细胞杂种植株的获得为选择具有酸橙优良性状、同时抗CTV的新型砧木提供了好的试材
Under the condition of known parameters, the embryogenic callus protoplasts of Citrus rusaurantium L. mesophyll protoplasts and C. sinensis Osbeckcv. Shamouti were induced by electric field. The fusion products were regenerated from 40 Plant. Chromosomal examination revealed that the resulting plants had 36 chromosomes and were tetraploid plants. Regenerated plants have wing, leaf thick, showing polyploid characteristics. Two 10-bp random primers were used to identify the hybrid characteristics of regenerated plants. In the amplified bands of two primers, the characteristic bands of the fused parents appeared in the random amplified bands of regenerated plants. Chromosome counting and RAPD analysis of regenerated plants showed that they are allochronic tetraploid somatic hybrid plants between lime and sweet orange. Obtaining of these somatic hybrid plants provides a good test material for selecting new rootstocks with excellent properties of lime and anti-CTV