A convenient and highly efficient strategy was developed for the simplification of enzymatic synthesis and purification of oligosac-charides.Glycosyl acceptors terminated with a thiol tag were extended by enzymatic modular assembly(EMA)in the aqueous phase,and the desired products were captured during solid-phase workup(SPW)using commercially available thiopropyl Sepharose 6B resin through a reversible disulfide linkage.Finally,the products were released from solid-phase resin in the presence of dithio-threitol(DTT)as reducing agent and the resin could be recovered and reused.This strategy overcomes the uncertain influence of polymer-or ionic-supports commonly used in enzymatic glycosylation,and showed perfect compatibility with all 10 enzyme mod-ules for the rapid assembly of a series of complex oligosaccharides.