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目的研究洛铂对宫颈癌SiHa细胞射线敏感性的影响及其机制。方法 MTT法检测不同浓度洛铂对SiHa细胞的作用24,48和72 h后的增殖情况,计算IC_(50)值。单克隆形成实验检测洛铂对SiHa细胞的射线敏感性影响,单击多靶模型拟合SiHa细胞的放射生物学参数。基于MTT法和细胞克隆形成实验结果,将细胞分为空白组(不加任何药物)和洛铂组(5.0μmol·L~(-1))、放疗组(6 Gy 6 MV X线)和洛铂+放疗组(5.0μmol·L~(-1)洛泊+6 Gy 6 MV X线)。流式细胞术检测洛铂、射线以及洛铂联合射线对SiHa细胞凋亡率的影响。Western blot检测促凋亡蛋白Bax和抑凋亡蛋白Bcl-2在SiHa细胞中的表达。结果空白组、洛铂组、放疗组和洛铂联合放疗组的凋亡率分别为(4.44±2.07)%,(30.50±3.17)%,(43.08±5.93)%和(84.68±4.90)%。洛铂组、放疗组相对空白组促凋亡蛋白Bax表达显著上调,抑凋亡蛋白Bcl-2显著下调。洛铂联合放疗组相对洛铂组、放疗组进一步显著上调Bax和下调Bcl-2的表达。结论洛铂可能通过细胞凋亡途径增强SiHa细胞射线敏感性。
Objective To investigate the effect of lobaplatin on the radiosensitivity of cervical cancer SiHa cells and its mechanism. Methods MTT assay was used to determine the proliferation of SiHa cells at various concentrations for 24, 48 and 72 h. IC 50 values were calculated. Monoclonal formation assay was used to detect the radiosensitivity effect of lobaplatin on SiHa cells. Click multi-target model to fit the radiobiological parameters of SiHa cells. The cells were divided into blank group (without any drug) and lobaplatin group (5.0μmol·L -1), radiotherapy group (6 Gy 6 MV X line) and Luo Platinum + radiotherapy group (5.0μmol·L -1 aporaphate + 6 Gy 6 MV X-ray). Flow cytometry was used to detect the effect of lobaplatin, radiotherapy and lobaplatin on the apoptosis of SiHa cells. Western blot was used to detect the expression of Bax and Bcl-2 in SiHa cells. Results The apoptotic rate of the blank group, lobaplatin group, radiotherapy group and lobaplatin combined with radiotherapy group were (4.44 ± 2.07)%, (30.50 ± 3.17)%, (43.08 ± 5.93)% and (84.68 ± 4.90)%, respectively. In the Losoplatin group and radiotherapy group, Bax expression was significantly up-regulated and Bcl-2 expression was significantly down-regulated in the blank group. In the combination of Losoplatin and radiotherapy, the radiotherapy group was significantly up-regulated Bax and down-regulated the expression of Bcl-2. Conclusion Losoplatin may enhance the radiosensitivity of SiHa cells through the apoptotic pathway.