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KNOX基因家族编码同源异型盒蛋白,在植物生长发育过程中起重要调控作用。利用生物信息学手段在全基因组水平上对大豆(Glycinemax)KNOX家族基因进行鉴定和分类,并分析其基因结构、蛋白同源结构域特征以及基因表达方式。研究结果表明:大豆中的27个GmKNOX基因可以分为GmKNOXI和GmKNOXII两个亚类,其中GmKNOXI类可分为3个主要的进化支,GmKNOXII类分为2个主要的进化支;26个GmKNOX基因不均匀地分布在16条染色体上,GmKNOX27尚无法定位。不同组织表达谱的分析表明:GmKNOXI类基因表达部位比较集中,以茎顶端分生组织中表达量最高;而GmKNOXII类基因的表达特异性较GmKNOXI类低,表达部位更广泛。
KNOX gene family encodes homeobox proteins that play an important regulatory role in plant growth and development. Bioinformatics methods were used to identify and classify Glycine max KNOX family genes at genome-wide level and to analyze their gene structure, protein homology domain characteristics and gene expression patterns. The results showed that 27 GmKNOX genes in soybean could be divided into two subgroups: GmKNOXI and GmKNOXII, of which GmKNOXI could be divided into three major evolutionary branches, GmKNOXII divided into two major evolutionary branches, 26 GmKNOX genes Unevenly distributed in 16 chromosomes, GmKNOX27 still can not locate. The analysis of the expression profiles of different tissues showed that the GmKNOXI genes were mostly expressed in the shoot apical meristem and the expression specificity of the GmKNOXII gene was lower than that in the GmKNOXI and the expression sites were more extensive.