论文部分内容阅读
目的 调查上海地区汉族、云南傣族和新疆维吾尔族 3个群体 MICA基因外显子 2、3和 4的多态性。方法 采用聚合酶链反应 -序列特异的寡核苷酸探针杂交 (polymerase chain reaction andsequence- specific oligonucleotide probing,PCR- SSOP)方法 ,分析 183名汉族、4 1名傣族和 6 6名维吾尔族正常人群的 MICA胞外区等位基因多态性。结果 分别在汉族、傣族和维吾尔族中检测出 10、7和 9个MICA等位基因 ,其中 MICA* 0 0 8在汉族和维吾尔族中频率最高 ,而傣族中 MICA* 0 10的频率最高。 3个民族 MICA等位基因分布方式各不相同 ,而且维吾尔族的等位基因分布与另外两个民族相比 ,差异具有显著性。结论 MICA等位基因分布方式具有民族地区特异性。
Objective To investigate the polymorphisms of exon 2, exon 2 and exon 4 of MICA gene in three populations of Han, Yunnan and Xinjiang Uygur in Shanghai. Methods A total of 183 Han, 41 Dai and 6 6 Uighur normal population were analyzed by polymerase chain reaction and sequence-specific oligonucleotide probing (PCR-SSOP) MICA extracellular region allele polymorphism. As a result, 10, 7 and 9 MICA alleles were detected in Han, Dai and Uygur ethnicity respectively. Among them, MICA * 0 0 8 had the highest frequency among Han and Uygur ethnic groups, while MICA * 0 10 had the highest frequency among Dai nationality. The distribution patterns of MICA alleles in 3 ethnic groups are different, and the distribution of alleles in Uygur nationality is significantly different from that of the other two ethnic groups. Conclusion The distribution of MICA alleles is ethnic-specific.