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对长江白甲鱼(Onychostoma sima)样本核DNA进行PCR扩增,获得白甲鱼核DNA上编码核糖体5.8SrRNA和28SrRNA基因的部分序列和完整的ITS2序列(876bp)。运用DNA分析软件对白甲鱼2个驯养群体(重庆水产研究所长寿湖珍稀鱼类繁育中心及涪陵鱼种场)进行了遗传多样性分析。结果表明:该序列平均T、C、A和G碱基组成为22%、32.5%、29.6%和15.8%,颠换Tv=40,转换Ts=10,转换和颠换比值为R=Ts/Tv=0.25。40个体都是单倍型,单倍型多样度为H=1.000,平均核苷酸差异系数K=5.978,核苷酸多样性Pi=0.0682。中性检验及聚类分析表明,两个群体没有分化成单一的群体,两个驯养群体遗传多样性高,种质质量良好。
The nuclear DNA of the Onychostoma sima sample was amplified by PCR and the partial sequence of ribosomal 5.8S rRNA and 28S rRNA gene and the complete ITS2 sequence (876bp) were obtained. DNA analysis software was used to analyze the genetic diversity of two domesticated white-turtle populations (Longevity Lake Rare Fish Breeding Center of Chongqing Institute of Fisheries and Fuling Fish Farm). The results showed that the average T, C, A and G base sequences of this sequence were 22%, 32.5%, 29.6% and 15.8%, the transposed Tv was 40 and the conversion was Ts = 10. The conversion and transversion ratios were R = Ts / Tv = 0.25.40 individuals were haplotype, haplotype diversity H = 1.000, the average nucleotide difference coefficient K = 5.978, nucleotide diversity Pi = 0.0682. Neutrality test and cluster analysis showed that the two groups did not differentiate into a single group, and the two domesticated groups had high genetic diversity and good germplasm quality.