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目的观测灰旱獭-长尾黄鼠鼠疫疫源地监测中应用酶联免疫吸附试验夹心法(ELISA)检测鼠疫F1抗体的效果,分析应用前景。方法 ELISA和间接血凝试验(IHA)同步检测动物血清鼠疫F1抗体,比较两种方法的阳性率、反应滴度。结果 ELISA和IHA检测血清的鼠疫F1抗体,长尾黄鼠阳性率分别为11.71%(118/1 008)和1.73%(21/1 216),平均滴度分别为27.805和21.254;牧犬阳性率分别为38.89%(28/72)和10.71%(7/75),平均滴度分别为27.731和21.50。ELISA检测长尾黄鼠、牧犬血清鼠疫F1抗体的阳性率和平均滴度均高于IHA,差异非常显著(P<0.001)。结论两种方法同步用于鼠疫监测,可及时发现并纠正单一方法因试剂变质或失效出现的错误结果,为制定防控措施提供准确的监测资料。
Objective To observe the effect of F1 antibody in plague flocks by using the enzyme-linked immunosorbent assay (ELISA) in the monitoring of the plague origin of the marmot - Nagymaros. Methods ELISA and indirect hemagglutination test (IHA) were used to detect the F1 antibody in serum of animals simultaneously. The positive rate and reaction titer of the two methods were compared. Results The positive rate of plague F1 antibody and long-tailed rat by ELISA and IHA were 11.71% (118/1 008) and 1.73% (21/1 216), respectively. The average titers were 27.805 and 21.254 respectively. 38.89% (28/72) and 10.71% (7/75) respectively, with the average titers of 27.731 and 21.50, respectively. The positive rate and the average titer of F1 antibody of the serum from the long-tailed Chlamys farrei and the mare were all higher than those from the IHA, the difference was significant (P <0.001). Conclusions The two methods are used simultaneously for the surveillance of plague. The single method can detect and correct the wrong result caused by deterioration or failure of reagents in time, and provide accurate monitoring data for the development of prevention and control measures.