【摘 要】
：
After harvest, cassava (Manihot esculenta Crantz) storage roots undergo rapid postharvest physio-logical deterioration, producing blue-brown discoloration in the vasculature due to the production of polyphenolics (mainly quinones and coumarins) by enzymes
【机 构】
：
National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sci
论文部分内容阅读
After harvest, cassava (Manihot esculenta Crantz) storage roots undergo rapid postharvest physio-logical deterioration, producing blue-brown discoloration in the vasculature due to the production of polyphenolics (mainly quinones and coumarins) by enzymes such as polyphenol oxidase (PPO). Here, we report the application of hen egg-white lysozyme (HEWL), a natural PPO inhibitor, in transgenic cassava to repress the symptoms of postharvest physiological de-terioration. The HEWL-expressing transgenic plants had lower levels of the two main cassava coumarins tested, scopoletin and scopolin, compared with wild type. HEWL-expressing cassava also showed increased tolerance of oxidative stress. Overall, the lysozyme-PPO system proved to be functional in plants for re-pressing PPO-mediated commercial product browning.
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