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目的探讨丹参保护兔缺血再灌注肾损伤的内皮机制。方法以兔肾缺血再灌注为模型 ,采用自动生化分析技术 ,检测不同实验条件下肾组织一氧化氮合成酶 (NOS)总活性及一氧化氮(NO) ,并用Elasa法测定尿视黄醇结合蛋白 (RBP)及尿微量白蛋白 (Alb) ,试图分析NOS总活性、NO与肾功能之间的相互关系。结果 (1)正常肾髓质较皮质能产生更多的NO。 (2 )缺血肾组织NOS总活性显著下降 ,NO显著增高。 (3)再灌注后 ,肾组织NOS总活性显著升高 ,而NO显著下降 ,肾功能明显受损。 (4 )肾缺血注射丹参后再灌注 ,肾组织NOS总活性进一步升高 ,而NO变化不明显 ,尿RBP及Alb下降至正常水平。结论 (1)生理情况下 ,肾髓质较皮质能产生更多的NO ,这对肾髓质血流分布及其功能的调节可能具有重要作用。 (2 )缺血再灌注后 ,肾组织NOS活性升高与肾功能受损密切相关。(3)丹参对缺血再灌注所致的肾小管及肾小球损伤均有明显的保护作用。 (4 )丹参对缺血再灌注肾组织中的NO具有抑制作用。
Objective To investigate the protective mechanism of salvia miltiorrhiza on endothelial dysfunction after ischemia-reperfusion in rabbits. Methods Rabbit kidney ischemia-reperfusion model was used to detect the total activity of nitric oxide synthase (NOS) and nitric oxide (NO) in renal tissue under different experimental conditions by automatic biochemical analysis. The urinary retinol was measured by Elasa method. RBP and urinary albumin (Alb) were attempted to analyze the relationship between total NOS activity, NO, and renal function. Results (1) Normal renal medulla produced more NO than the cortex. (2) The total NOS activity in ischemic kidney tissue decreased significantly, and NO increased significantly. (3) After reperfusion, the total activity of NOS in kidney tissue increased significantly, while NO significantly decreased, and renal function was significantly impaired. (4) After renal ischemic injection of Salvia miltiorrhiza, the total activity of NOS in renal tissue was further increased, while NO was not changed significantly. Urinary RBP and Alb decreased to normal levels. Conclusions (1) Under physiological conditions, the medullary medulla produces more NO than the cortex, which may play an important role in regulating the distribution and function of the blood flow of the renal medulla. (2) After ischemia and reperfusion, the increase of NOS activity in renal tissue is closely related to the impaired renal function. (3) Salvia miltiorrhiza has obvious protective effect on renal tubular and glomerular injury caused by ischemia-reperfusion. (4) Salvia miltiorrhiza has inhibitory effect on NO in ischemia-reperfusion kidney tissue.