一、引言 显微全息术是显微和全息相结合的技术,其特点是将微小物体通过显微技术进行放大,通过全息技术进行三维信息贮存,这两种技术的先后次序是可以互换的,因此显微全息术分两种类型,一种是用显微术将标本图像放大再记录其全息图称预放大;另一种是先拍摄标本的全息图再用显微镜进行观察称后放大。显微全息术最突出的优点是可以拍摄生物活标本的瞬态全息图,便于进行三维观察或“冻结”它的某个瞬态进行长期保存。这种情况通常需使用脉冲激光器或高功率连续激光器,这些光源价格昂贵,而且由于照射在活标本上的激光束功率密度高,往往会影响甚至破坏它的正常生长。本文介绍的一种显微全息光路,它无需庞大的防震 I. Introduction Micro-holography is a combination of microscopy and holography technology, which is characterized by the microscopic objects through the magnification of the three-dimensional information through holographic storage technology, the order of the two technologies are interchangeable Therefore, microscopic holography divided into two types, one is the use of microscopy will enlarge the specimen and then record the hologram pre-amplification; the other is the first shot of the hologram of the specimen and then observed by a microscope scale and then enlarged. The most prominent advantage of microscopic holography is the ability to take transient holograms of biological specimens for long-term preservation of one of its transients for three-dimensional observation or “freezing”. This usually requires the use of pulsed lasers or high-power continuous lasers, which are expensive and often have a detrimental effect on their normal growth due to the high power density of the laser beam impinging on the specimen. This article describes a microscopic holographic optical path, which does not require a large shock