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目的对乙脑减毒活疫苗生产用毒种即乙脑病毒SA14-14-2减毒株的现行主种子批、工作种子批进行分枝杆菌检测,更好地控制外源因子对乙脑减毒活疫苗生产用毒种的污染。方法运用改良罗氏培养基培养法对乙脑病毒SA14-14-2减毒株的4个批号毒种样本进行分枝杆菌检测。结果 4个批号的乙脑病毒SA14-14-2减毒株毒种样本(主种子批,批号:9903;工作种子批,批号:JEV-W-7-20120701、JEV-W-7-20120702及JEV-W-7-20131201)的分枝杆菌培养结果无菌落生长。结论本次试验中4个批号的乙脑病毒SA14-14-2减毒株毒种样本分枝杆菌培养检测为阴性,说明乙脑毒种样本没有受到分枝杆菌污染,进而保障了乙脑减毒活疫苗的安全性。
OBJECTIVE To detect mycobacteria in the current main seed batch and working seed batch of the attenuated strain of JE virus attenuated strain of JE virus, and to better control the effect of exogenous factor on JE Toxic live vaccine production with poisonous species. Methods Mycobacterium tuberculosis was detected in four batches of virus strains of attenuated strains of JE-SA14-14-2 by modified Roche culture method. Results Four batches of JEV SA14-14-2 attenuated strain samples (main seed batch, batch number: 9903; working seed batch, batch number: JEV-W-7-20120701, JEV-W-7-20120702 and JEV-W-7-20131201) mycobacterial culture results colony growth. Conclusions In this experiment, four batches of mycobacterial SA14-14-2 attenuated strains of Mycobacterium avium samples were tested negative for mycobacterial culture, indicating that mycotoxin samples were not contaminated by mycobacteria, Safety of live vaccine.