论文部分内容阅读
本实验给大鼠一次静脉注射~(14)4C-甲基苄基亚硝胺(~(14)C-NMBzA,2.5mg/kg)后3～4小时处死,用高效液相色谱仪及液体闪烁计数器分离和测定大鼠食道等组织的DNA烷基化程度。结果发现饮乌龙茶和茉莉花茶2～3周的大鼠,食道上皮细胞DNA中的O~6-甲基鸟嘌呤(O~6-meG)含量呈不同程度的下降,为茶叶防癌作用机理的探讨提供了依据。
In this study, rats were sacrificed 3-4 hours after intravenous injection of ~ (14) 4C-methylbenzylnitrosamine (~ (14) C-NMBzA, 2.5 mg / kg) using high performance liquid chromatography Scintillation counter separation and determination of rat esophagus and other organizations DNA alkylation. The results showed that the contents of O ~ 6-methylguanine (O ~ 6-meG) in the esophageal epithelial cells of rats after 2 ~ 3 weeks of drinking oolong tea and jasmine tea decreased to some extent, which was the mechanism of anti-cancer effect of tea Provide a basis for discussion.