Land use and land cover (LULC) changes strongly affect local hydrology and sediment yields. The current study focused on a basin in the Brazilian Amazon and had the following three objectives:(1) to perform an effective diagnosis of flow and sediment yield, (2) to evaluate the impacts of LULC changes over the last 40 years on the hydro-sedimentological variables, and (3) to investigate the impacts of the possible trends or breaking points in the flow, surface runoff, and sediment yield series. The Soil and Water Assessment Tool (SWAT) model validation showed that the simulated results were consistent with the data measured in the dynamic reproduction of flow seasonality. Furthermore, changes in LULC altered surface runoff, sediment yield, and flow according to the Mann-Kendall and Pettitt non-parametric tests. It was also observed that the sub-basins in which pastureland is pre-dominant are more susceptible to increased surface runoff and sediment yield. According to trend and homogeneity tests, these sub-basins had break points in the time series of these variables. On the other hand, in the sub-basins whose land cover is predominantly forest, the time series is homoge-neous and trendless. The current study highlighted the impacts that 40 years of LULC changes in an Amazonian basin had on hydro-sedimentological variables, which, in turn, can play an important role in changing the hydrological cycle, consequently these changes can also impact diverse human ac-tivities in the region, such as agribusiness, livestock, energy production, food security, and public water supply. Thus, it is concluded that the SWAT model is a good estimator of hydro-sedimentological processes in Amazonian basins and can be used by decision makers in the management of water and environmental resources.