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目的:探索一氧化氮合酶(NOS)阳性神经元在便秘型肠易激综合征(C-IBS)大鼠结肠的分布特点及变化。方法:冷水持续灌胃15 d制作C-IBS大鼠模型。通过对大鼠大便性状和结肠内膨胀刺激后腹肌反应的观察及结肠HE染色对模型进行评价。免疫荧光组织化学和Western Blot观察NOS阳性神经元在结肠肠肌丛的分布及改变。结果:造模15 d后,模型组大鼠大便呈腊肠状的碎块,结肠的腹肌反应阈值明显降低,HE染色显示结肠粘膜层完整。免疫荧光组织化学显示,C-IBS大鼠结肠肠肌从神经元NOS表达较对照组明显增高。Western Blot结果显示,C-IBS大鼠结肠NOS的表达明显增加。结论:NO在C-IBS大鼠结肠神经元内表达增加,此异常可能与C-IBS大鼠结肠运动功能紊乱相关。
Objective: To investigate the distribution and changes of nitric oxide synthase (NOS) positive neurons in the colon of constipation-prone irritable bowel syndrome (C-IBS) rats. Methods: C-IBS rat model was established by intragastric administration of cold water for 15 days. The model was evaluated by observing the abdominal reaction and the colon HE staining after the stool of the rats and the colon. Immunofluorescence histochemistry and Western Blot were used to observe the distribution and changes of NOS positive neurons in the colon and myenteric plexus. Results: After 15 days of modeling, the defecation of the model group was sausage-like fragments, the reaction threshold of the colon decreased significantly, and the colon mucosa was intact by HE staining. Immunofluorescence histochemistry showed that NOS expression in colonic muscle of C-IBS rats was significantly higher than that in control group. Western Blot results showed that the expression of NOS in C-IBS rats increased significantly. CONCLUSION: NO is increased in colonic neurons of C-IBS rats, and this abnormality may be related to colonic motor dysfunction in C-IBS rats.