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目的 为提高医院感染细菌培养阳性率及抗菌药物的有效使用。方法 对我院 1995年 1月~ 1997年 12月从 1870例住院患者采取血、尿、脑脊液、胸腹水、胆汁等标本进行了常规与高渗同步培养的结果进行了对照分析。结果 分离出 L型细菌 4 2 7株 ,而普通培养均为阴性 ,阳性率提高了 2 2 .8% ,分离菌种达 11种之多 ;以金黄色葡萄球菌在各类标本中感染率占首位 ,19.4 4% ;大肠埃希菌占第 2位 ,16.4 % ,凝固酶阴性葡萄球菌、D群肠球菌、酵母菌占第 3位 ,分别为 11%、10 .77%、10 .3 % ;其它各菌属均占有一定比例。对青霉素、头孢类抗生素高度耐药 ,对红霉素、氯霉素、庆大霉素、林可霉素呈低度耐药。结论 采取同步培养可提高阳性率 ,为临床感染性疾病诊断和抗生素的合理使用提供了重要依据。
The purpose is to improve the positive rate of nosocomial bacterial culture and the effective use of antibacterials. Methods A total of 1870 inpatients from January 1995 to December 1997 in our hospital were enrolled in the routine and hypertonic synchronous culture of blood, urine, cerebrospinal fluid, pleural, ascites and bile samples. Results Four hundred and seventy-seven strains of L-type bacteria were isolated, but all of them were negative in normal culture. The positive rate was increased by 22.8% and the number of isolates was 11. Staphylococcus aureus infection in various specimens accounted for The first place, 19.4%; Escherichia coli accounted for the second place, 16.4%, coagulase-negative Staphylococcus aureus, D group Enterococcus, yeast accounted for the third place, respectively 11%, 10.77%, 10.3% ; Other bacteria are occupying a certain percentage. Of penicillin, cephalosporins highly resistant to erythromycin, chloramphenicol, gentamicin, lincomycin was low resistance. Conclusion The simultaneous culture can improve the positive rate, which provides an important basis for the diagnosis of clinical infectious diseases and the rational use of antibiotics.