论文部分内容阅读
目的:探讨温度对动物尸体内HbCO含量的影响,为火灾中死亡尸体血中HbCO含量的判断提供参考依据。方法:CO中毒死亡大鼠和对照组大鼠尸体分别在室温25℃放置及100℃、150℃、200℃加热10 min和15 min后取心血检测其HbCO含量。实验家兔在常温下通入CO致中毒死亡,抽取对照组家兔和CO中毒死后家兔心血,分别在室温25℃放置3 min及100℃、150℃、200℃加热3 min后紫外-可见分光光度法检测其HbCO含量。结果:CO中毒死后的家兔血液中HbCO含量随着温度的升高呈下降趋势;CO中毒死亡大鼠尸体心血中HbCO含量随着温度升高和加热时间延长呈下降趋势。结论:CO中毒死亡后的动物尸体血液中HbCO含量会随温度及加热时间的改变而发生变化。
Objective: To investigate the influence of temperature on the content of HbCO in animal carcasses, and to provide a reference for judging the content of HbCO in dead cadaver blood during a fire. Methods: The body mass of rats killed by CO poisoning and the control group were respectively measured at 25 ℃ for 10 min and 15 min at 100 ℃, 150 ℃ and 200 ℃ for determination of HbCO content. The rabbits were poisoned with CO at room temperature. The rabbits in the control group and the rabbits after the poisoning of CO were drawn out and centrifuged at 25 ℃ for 3 min and at 100 ℃, respectively. After heating at 150 ℃ and 200 ℃ for 3 min, Visible spectrophotometry to detect its HbCO content. Results: The content of HbCO in the blood of rabbits after CO poisoning decreased with the increase of temperature. The content of HbCO in the blood of deceased rats with CO poisoning decreased with the increase of temperature and prolongation of heating time. Conclusion: The HbCO content in the dead animal body after CO poisoning changes with the change of temperature and heating time.