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紫外分光光度法在药物分析中的应用已极为普遍。本文仅讨论几种不需特殊仪器、不经分离,通过适当数学处理以消除干扰吸收的光谱测定法。一、联立方程法联立方程法是根据吸收度的加和性原理,在吸收光谱重叠的两组分的最大吸收波长处分别测定混合物的吸收度。再建立联立方程求解后则可得到各组分的含量。例如:银黄注射液中有效成分绿原酸和黄芩甙的最大吸收波长分别为324nm和280nm。二者的吸收光谱重叠。在这二个最大吸收波长处分别测定混合液的吸收度,建立联立方程,求解后得绿原酸和黄芩甙的含量。该法的特点是操作简便,重现性好。但是必须知道各待测组分在各测定波长处的吸收系数。这使该法在中药分析中的应用受到限制。
The application of UV spectrophotometry in drug analysis has become extremely common. This article only discusses several spectrometric methods that do not require special instruments and are not separated, and that eliminate interference absorption by proper mathematical processing. 1. Simultaneous equation method The simultaneous equation method is based on the additive principle of absorbance. The absorbance of the mixture is measured at the maximum absorption wavelength of the two components where the absorption spectrum overlaps. After the simultaneous equations are established, the content of each component can be obtained. For example, the maximum absorption wavelengths of chlorogenic acid and baicalin in the active ingredient of Yinhuang Injection are 324 nm and 280 nm, respectively. The absorption spectra of the two overlap. The absorbance of the mixture was measured at the two maximum absorption wavelengths, and simultaneous equations were established to obtain the content of chlorogenic acid and jaundice. The method is characterized by simple operation and good reproducibility. However, it is necessary to know the absorption coefficient of each component to be measured at each measurement wavelength. This limits the application of this method in the analysis of traditional Chinese medicine.