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目的:采用高效液相色谱-飞行时间质谱(HPLC-TOF/MS)联用技术快速鉴定远志生、制饮片的化学成分,探讨远志炮制前后化学成分的变化规律。方法:高效液相色谱采用HaloC18色谱柱(2.1 mm×100 mm,2.7μm),流动相乙腈-0.1%甲酸水溶液梯度洗脱;质谱使用电喷雾离子源,在正离子模式下扫描,获得远志生、制饮片总离子流图和离子峰质谱数据;通过对照品定位、质谱数据和文献参照对各离子峰进行归属,比较远志炮制前后离子峰数目和峰强度的变化。结果:从生远志中鉴定了21个化合物,其中13种寡糖酯类成分,8种皂苷类成分。从制远志中鉴定了20种化合物,其中11种寡糖酯类成分,9种皂苷类成分。远志经甘草汁煮制后,tenuifoliose C或tenuifoliose E,tenuifoliose K的分子离子峰消失,而西伯利亚远志糖A5,西伯利亚远志糖A6,tenuifoliside A,tenuifoliside B,tenuifoliside C,3,6’-二芥子酰基蔗糖,tenuifoliose A,tenuifoliose B或tenuifoliose D,tenuifoliose H,远志皂苷Vg,远志皂苷Wg及远志皂苷Gg的分子离子峰强度显著降低,且产生新成分细叶远志皂苷。结论:远志经甘草汁煮制后,部分寡糖酯类成分和多种皂苷类成分含量变化显著。
OBJECTIVE: To rapidly identify the chemical constituents of Polygalaceae and Pieces by high performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF / MS), and to study the chemical composition changes of Polygala. Methods: High performance liquid chromatography was performed on a Halo C18 column (2.1 mm × 100 mm, 2.7 μm) with a mobile phase of acetonitrile-0.1% formic acid in aqueous solution. The mass spectra were obtained by electrospray ionization and scanning in positive mode Polygala tenuifolia, the total ion current flow chart and ion peak mass spectrometry data; through the reference substance mapping, mass spectrometry data and literature reference to the ion peak assignment, compared Polygala before and after processing the number of ion peaks and peak intensity changes. Results: 21 compounds were identified from Sheng Yuan Zhi, including 13 kinds of oligosaccharides and 8 kinds of saponins. Twenty compounds were identified from Polygala tenuifolia, including 11 kinds of oligosaccharide esters and 9 kinds of saponins. Polygala tenuifoliose C or tenuifoliose E, tenuifoliose K molecular ion peak vanished after Polygala tenuifoliose cooked by licorice juice, while the Siberian Polygala tenugar A5, Siberian Polygala tenugar A6, tenuifoliside A, tenuifoliside B, tenuifoliside C, 3,6’- The molecular ion peak intensities of sucrose, tenuifoliose A, tenuifoliose B or tenuifoliose D, tenuifoliose H, teigenin Vg, teigenin Wg and teinosaponin Gg were significantly reduced, and the new component of Polygalaceae saponin was produced. Conclusion: After Polygalaceae licorice juice is prepared, the contents of some oligosaccharides esters and various saponins vary significantly.