对低能氮离子注入甜菊种子引起的生长发育、细胞核和染色体形态结构的影响进行研究 .结果表明 ,能量为 75Ke V、剂量为 10 14 / cm2的氮离子束注入甜菊种子后 ,种子萌发率下降 ;幼苗期生长迟缓 ;细胞分裂指数下降 ,异常细胞核和染色体畸变增加 ,细胞核膜凹凸不平 ,核仁解聚 ,部分核质凝集并被凹陷的核膜包被成颗粒化小区 ,进而形成微核 ,细胞核的形态建成和细胞分裂受到一定的干扰 .推测这些现象与幼苗期处理种子的早期生长发育迟缓有关 . The effects of low energy nitrogen ion implantation on the growth and development, nuclear and chromosomal structure induced by the seeds of Stevia rebaudiana were studied.The results showed that the seed germination rate decreased with the implantation of stevia seeds at a dose of 10 14 / cm2 with energy of 75 KeV, Seedling growth retardation; cell division index decreased, abnormal nuclei and chromosome aberrations increased, the nuclear membrane irregularities, nucleolus depolymerization, part of the nucleolus coagulation and the hollow nuclei were coated into granular cells, and then the formation of micronuclei, nuclei The formation of morphology and cell division were some interference.It is speculated that these phenomena are related to the early growth retardation of seed treatment of seedling.