【摘 要】
：
LIN28A,an RNA-binding protein,plays an important role in porcine induced pluripotent stem cells(piPSCs).However,the molecular mechanism underlying the function
【机 构】
：
College of Veterinary Medicine,Shaanxi Centre of Stem Cells Engineering and Technology,Northwest A&F
论文部分内容阅读
LIN28A,an RNA-binding protein,plays an important role in porcine induced pluripotent stem cells(piPSCs).However,the molecular mechanism underlying the function of LIN28A in the maintenance of pluripotency in piPSCs remains unclear.Here,we explored the function of LIN28A in piPSCs based on its overexpression and knockdown.We performed total RNA sequencing(RNA-seq)of piPSCs and detected the expression levels of relevant genes by quantitative real-time polymerase chain reaction(qRT-PCR),western blot analysis,and immunofluorescence staining.Results indicated that piPSC proliferation ability decreased following LIN28A knockdown.Furthermore,when LIN28A expression in the shLIN28A2 group was lower(by 20％)than that in the negative control knockdown group(shNC),the pluripotency of piPSCs disappeared and they differentiated into neuroectoderm cells.Results also showed that LIN28A overexpression inhibited the expression of DUSP(dual-specificity phosphatases)family phosphatases and activated the mitogen-activated protein kinase(MAPK)signaling pathway.Thus,LIN28A appears to activate the MAPK signaling pathway to maintain the pluripotency and proliferation ability of piPSCs.Our study provides a new resource for exploring the functions of LIN28A in piPSCs.
其他文献
In domestic dogs Canis familiaris,vocal traits have been investigated for barks and growls,and the relationship between individual body size and vocal traits in
摘要：宋国生《二胡基本功教学用语中的几个关键词》发表于2008年《天津音乐学院学报》第3期，作者在文中共提出了七个基本功教学用语，这七个关键词既准确又生动形象地概括了二胡演奏中所涉及的基本功理论知识，分别从关键词的含义、动作要点、动作原理、训练方法以及注意事项等几个方面，对七个关键词进行深入的解释和分析。本文将“七个关键词”作为主要研究内容，通过阐述笔者对“七个关键词”的理解，结合个人学习心得和演
摘要：欧内斯特·肖松（Amédée Ernest Chausson，1855年—1899年），是19世纪浪漫主义中后期对法国音乐的发展有着重要作用的作曲家。肖松师从弗兰克，崇拜瓦格纳，并与德彪西交好。他的音乐风格既有浪漫主义晚期打破调性的倾向，又有印象主义色彩性音响的特色。本文对肖松晚期作品《音诗》（Poe?me，op.25）中和声、曲式进行探析，研究肖松在该作品中作曲技法的继承与创新。 关键词
气隙式热开关是空间深低温系统应用中的关键技术,需针对氦介质气隙式热开关的转变温度特征开展研究.基于稀薄气体传热理论,建立了不同充气压力和间隙尺寸对热开关转变温度影
为探索两级汽液分离CO2引射制冷系统中喷嘴第一喉部直径对其性能的影响,实验测量了使用不同喷嘴第一喉部直径的两级汽液分离CO2引射制冷系统的性能,并与传统CO2两级节流引射
A multilayer network approach combines different network layers,which are connected by inter-layer edges,to create a single mathematical object.These networks c
高温超导复合缆线的运行温度直接决定其载流性能,超导带材交流损耗计算的准确性对缆线的温升计算至关重要.面向具有铜骨架和双层高温超导带材结构的高温超导复合缆线,通过改
在高压直流输电系统中,高温超导直流电缆具有高功率密度特点被广泛使用.然而,电网中的直流以及开关器件和环境产生的交流纹波,都造成交流损耗.由第二代(2G)带材组成的五边形
针对传统的飞机地面空调车存在不能以小功率用电保障大功率需求及冷量损失严重等问题,提出一种相变蓄冷式飞机地面空调车,简要分析了系统组成及其原理,对蓄、释冷系统进行了
基于二维轴对称H方程法对用于高温超导脉冲变压器的YBCO双饼线圈进行了有限元建模与仿真,研究了分磁环参数和开槽数对YBCO双饼线圈传输原边电流时的磁场特性和交流损耗的影响