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Objective: To demonstrate the anti-inflammatory activity of Brassica napus L.hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.Methods: Composition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were extracted.The nitric oxide (NO) production was measured using the Griass assay.Prostaglandin E2 (PGE2) production was evaluated with enzyme-linked immunosorbent assay.The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Westem blot analysis.Furthermore,phosphorylation of nuclear factor-kappa B (NF-κ B) and nuclear translocation of NF-κ B p65 were evaluated with Wastem blot analysis and immunofluorascence staining,respectively.Results: Compared with LPS-stimulated cells,BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P＜0.01 or P＜0.05).Moreover,BNH inhibited protein levels of iNOS and COX-2 (P＜0.01).Phosphorylation of NF-κ B and nuclear translocation of NF-κ B p65 was significantly inhibited by BNH (P＜0.01or P＜0.05).Conclusion: The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.