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目的探讨凝血酶预处理(thrombin preconditioning,TPC)对大鼠脑出血后内源性的神经细胞再生的影响。方法 SD大鼠随机分为对照组、脑出血(Intracerebral hemorrhage,ICH)组和TPC组,每组分为3、7、14、21、28 d亚组。应用胶原酶脑内立体定向注射制作脑出血模型。TPC组首先将1U凝血酶立体定向注入右侧纹状体,1 d后再制作脑出血模型。所有大鼠应用Brd U腹腔注射在体标记再生的脑室下带(subventricular zone,SVZ)细胞,应用免疫组织化学技术检测Brd U阳性细胞。结果脑出血后3 d同侧SVZ和基底节Brd U阳性细胞开始增加,但与对照组比较差异没有显著性(P>0.05),7 d时Brd U阳性细胞数增加明显,与对照组比较有明显差异(P<0.05),14 d达到高峰,然后逐渐下降,28 d时Brd U阳性细胞虽然仍有增加,但与对照组比较差异没有显著性(P>0.05)。TPC组3 d时Brd U阳性细胞数目开始增加,与脑出血组和对照组比较,差异均具有显著性(P<0.01),14 d达高峰,一直持续至21 d,28 d时Brd U阳性细胞虽然略有下降,但与对照组和脑出血组比较,仍有统计学差异(P<0.05)。结论凝血酶预处理能够增强脑出血后内源性神经再生,可能为脑出血后内源性神经再生的研究提供新的思路。
Objective To investigate the effect of thrombin preconditioning (TPC) on the regeneration of endogenous nerve cells after intracerebral hemorrhage in rats. Methods SD rats were randomly divided into control group, Intracerebral hemorrhage (ICH) group and TPC group. Each group was divided into subgroups 3, 7, 14, 21 and 28 d. Intrathecal injection of collagenase intracerebral stereotaxic injection of intracerebral hemorrhage model. TPC group first stereotactic injection of 1U thrombin into the right striatum, 1 d before making a model of intracerebral hemorrhage. All rats were intraperitoneally injected with Brd U in vivo and labeled with subventricular zone (SVZ) cells. Immunohistochemistry was used to detect BrdU positive cells. Results The ipsilateral SVZ and basal ganglia BrdU positive cells started to increase 3 days after ICH, but there was no significant difference compared with the control group (P> 0.05), and the number of BrdU positive cells increased significantly on the 7th day, compared with the control group (P <0.05), and reached the peak on the 14th day, then gradually decreased. At 28th day, the number of BrdU positive cells was still increased, but there was no significant difference compared with the control group (P> 0.05). The number of BrdU positive cells began to increase on day 3 in TPC group, and there was a significant difference between cerebral hemorrhage group and control group (P <0.01), reaching the peak on day 14 and continuing until 21 d. On the 28th day, BrdU positive Although the cells decreased slightly, there was still a significant difference compared with the control group and the cerebral hemorrhage group (P <0.05). Conclusion Thrombin preconditioning can enhance endogenous nerve regeneration after intracerebral hemorrhage and may provide new ideas for the study of endogenous nerve regeneration after intracerebral hemorrhage.