论文部分内容阅读
目的:评价雷公藤内酯对海人酸致痫大鼠神经元的免疫保护作用。方法:结晶紫染色观察神经元形态,免疫组化技术检测小胶质细胞MHC-Ⅰ、Ⅱ类分子的表达。结果:30μg/kg雷公藤内酯可使大鼠的癫痫抽搐状态明显较海人酸组缓和,出现时间较海人酸组晚(P<0.05),且可使海人酸诱导大鼠活化小胶质细胞的数量减少,凋亡神经元数量减少。30μg/kg雷公藤内酯可下调海人酸诱导大鼠脑小胶质细胞表达MHC-Ⅰ、Ⅱ类分子(P<0.01)。结论:雷公藤内酯通过抑制小胶质细胞表达MHC-Ⅰ、Ⅱ类分子和免疫应答对海人酸诱导大鼠神经元凋亡具有免疫保护作用。
Objective: To evaluate the protective effect of triptolide on neurons in epileptic rats induced by kainate. Methods: The morphology of neurons was observed by crystal violet staining. The expression of MHC-Ⅰ and Ⅱ molecules in microglia was detected by immunohistochemistry. Results: Triptolide at 30 μg / kg significantly delayed the seizure status of epileptic seizures in rats compared with that of the kainate group (P <0.05), and delayed the onset of seizures The number of stromal cells decreased and the number of apoptotic neurons decreased. Triptolide at 30μg / kg could downregulate the expression of MHC-Ⅰ and Ⅱ molecules in rat microglia cells induced by kainate (P <0.01). It is concluded that triptolide has immunoprotective effects on the neuronal apoptosis induced by kainate in the microglia cells by inhibiting the expression of MHC class I and class II molecules and immune responses.