用制备性ＳＤＳ丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）分离空肠弯曲菌Ｓ１３１株的菌体蛋白。在ＫＣｌ“染色背景下，切下６５．４７Ｋｕ蛋白带，经电泳洗脱和浓缩获得的蛋白液，免疫印迹证实为均一鞭毛蛋白．另用亚硝基胍诱导使空肠弯曲菌Ｓ１３１有动力株突变为无动力株，电镜下突变株鞭毛消失，但ＳＤＳ－ＰＡＧＥ谱上仍有６５．４７Ｋｕ鞭毛蛋白区带。提示，这种鞭毛突变可能发生在蛋白质装配阶段。 The bacterial proteins of Campylobacter jejuni strain S131 were separated by preparative SDS acrylamide gel electrophoresis (SDS-PAGE). Under the background of KCl staining, the 65.47Ku protein band was excised, and the obtained protein solution was eluted and concentrated by electrophoresis, and the immunoblot was confirmed to be uniform flagellin.In addition, nitrosoguanidine was used to induce mutant of Campylobacter jejuni S131 However, there were still 65.47Ku flagellin bands in the SDS-PAGE, suggesting that this flagella mutation may occur during the protein assembly stage.