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目的研究1.8GHz(比吸收率为3W/kg)微波(MW)对紫外线(UV)诱发的人外周淋巴细胞DNA损伤的影响。方法淋巴细胞采自3名健康青年,体外实验暴露波长为254nm的UV,剂量分别为0.25、0.50、0.75、1.00、1.50和2.00J/m~2;微波暴露0、1.5、4.0h。并设UV与MW的联合暴露组,处理后淋巴细胞再培养0、1.5和4.0h,用彗星试验检测淋巴细胞DNA损伤情况。结果MW组所诱发的DNA损伤与对照组相比,差异无统计学意义(P>0.05);UV所诱发的DNA损伤(平均尾长MTL)分别为(1.71±0.09)、(2.02±0.08)、(2.27±0.17)、(2.27±0.06)、(2.25±0.12)和(2.24±0.11)μm,明显高于空白对照组[(0.96±0.05)μm],差异有统计学意义(P<0.01)。UV与MW的联合暴露组所诱发的MTL,在培养1.5h时,部分剂量组低于相应的UV组;在培养4h时,部分剂量组高于相应的UV组,差异均有统计学意义(P<0.01或P<0.05)。结论1.8GHz微波暴露1.5～4.0h后未见人淋巴细胞DNA损伤增加,但经不同的培养时间,能增强或降低UV所诱发的DNA损伤效应。
Objective To investigate the effect of microwave (MW) at 1.8GHz (specific absorption rate of 3W / kg) on DNA damage induced by ultraviolet (UV) in human peripheral blood lymphocytes. Methods Lymphocytes were collected from three healthy young volunteers. The experimental UV exposure wavelength was 254 nm. The doses were 0.25, 0.50, 0.75, 1.00, 1.50 and 2.00 J / m ~ 2, respectively. The microwaves were exposed for 0, 1.5 and 4.0 h. Combined UV and MW exposures were set. Lymphocytes were cultured for 0, 1.5 and 4.0 h after treatment. DNA damage of lymphocytes was detected by comet assay. Results There was no significant difference in the DNA damage induced by MW group compared with the control group (P> 0.05). The DNA damage induced by UV was (1.71 ± 0.09) and (2.02 ± 0.08) (2.27 ± 0.17), (2.27 ± 0.06), (2.25 ± 0.12) and (2.24 ± 0.11) μm, respectively, which were significantly higher than those in the blank control group [(0.96 ± 0.05) μm] ). The MTL induced by UV and MW exposure group was lower than that of corresponding UV group at 1.5h after culture, and some dose groups were higher than corresponding UV group at 4h P <0.01 or P <0.05). Conclusion No DNA damage of human lymphocytes was observed after exposure to 1.8GHz microwave for 1.5-4.0h, but DNA damage induced by UV could be enhanced or decreased by different culture time.