Four-point flexural fatigue test for Gussasphalt mixture specimen was carried out at a strain-controlled mode system. The results showed that the development of the tested stiffness modulus and phase angle of the mixtures with increasing load cycles exhibited three periods, initial generation, slow development and failure period. The fatigue crack generation zone formed in the third period, in which the macro mechanical properties were significantly decreased. Moreover, we also analyzed the effects of asphalt content and mixing temperature on the fatigue life of the mixture. The results showed that the first period when the specimen’s initial stiffness modulus was reduced to 80%accounted for 5%-10%of the total fatigue life;the second period in which the reduction became slow and demonstrated a liner relationship with load cycles occupied 70%-85%of the fatigue life;and the third period was about 5%-10%. The results indicated that the lower the mixing temperature, the longer the fatigue life of Gussasphalt mixture. Besides, the increasing of asphalt content has a minor effect on the fatigue life of Gussasphalt mixture