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目的研究浒苔(绿藻门,石莼科,浒苔属)正丁醇萃取相的化学成分及其对CCl4和D-GalN诱导的小鼠肝损伤的保护作用。方法采用硅胶柱色谱、Sephadex LH-20柱色谱、高效液相色谱、薄层制备等方法进行分离纯化;利用MS、1 H NMR、13 C NMR波谱分析结合文献对照鉴定化合物结构;用CCl4和D-GalN诱导小鼠肝损伤造模,检测小鼠肝脏指数,血清中AST、ALT水平,肝脏中总SOD、MDA水平来探讨其对肝损伤的保护作用。结果从浒苔正丁醇萃取相分离鉴定出了6种化合物:对羟基苯甲酸甲酯,2-脱氧尿嘧啶核苷,尿苷,尿嘧啶,次黄嘌呤,腺嘌呤;活性实验方面,与模型组相比,中剂量组、高剂量组均可以显著抑制肝脏指数,血清AST、ALT以及肝脏中MDA的水平,各剂量组对受损肝脏SOD水平都有明显的恢复作用。结论浒苔正丁醇相化学成分以核苷类化合物为主,而且可以明显减轻由CCl4和D-GalN诱导的小鼠急性肝损伤。
Objective To study the chemical constituents of n-butanol extraction phase of Enteromorpha (Chlorophyta, Ephemeralidae, Enteromorpha) and their protective effects on liver injury induced by CCl4 and D-GalN in mice. Methods The compounds were isolated and purified by silica gel, Sephadex LH-20 column chromatography, high performance liquid chromatography and thin layer chromatography. The structure of compounds were identified by MS, 1H NMR and 13 C NMR spectroscopy. -GalN induced hepatic injury in mice, detected the liver index, serum AST, ALT levels, liver total SOD, MDA levels to explore its protective effect on liver injury. Results Six compounds were identified from the n-butanol extraction phase of Enteromorpha prolifera: methylparaben, 2-deoxyuridine, uridine, uracil, hypoxanthine and adenine; in the activity experiment, Compared with model group, middle dose group and high dose group could significantly inhibit liver index, serum AST, ALT and liver MDA levels, and all dose groups had obvious recovery on the level of SOD in liver. Conclusion The n-butanol phase of Enteromorpha is dominated by nucleosides, and the acute liver injury induced by CCl4 and D-GalN can be significantly alleviated.