论文部分内容阅读
利用综合隶属函数法评价不同产地8个花生品种幼苗的抗旱能力,同时分析各品种花生在模拟干旱处理2小时和12小时后叶片AhNCED1基因表达量的变化,探讨花生品种抗旱性与叶片AhNCED1基因表达的关系,建立花生(Arachis hypogaea)幼苗时期通过AhNCED1基因表达变化检测花生抗旱性等级的方法。结果显示,干旱处理2小时,各品种叶片AhNCED1基因表达量迅速增加,与处理前相比差异极显著;干旱处理12小时,各品种花生叶片AhNCED1基因表达量均降低,但仍均高于处理前水平。综合隶属函数值定量反映各品种抗旱性强弱顺序为:花育24号>福花13号>粤油7号>中花15号>中花16号>航花2号>福花9号>北海1号。干旱处理2小时各品种花生叶片AhNCED1表达量变化大小依次为:福花13号>花育24号>中花16号>中花15号>航花2号>粤油7号>北海1号>福花9号。其中,抗旱性强的品种为花育24号和福花13号,抗旱性弱的品种为福花9号和北海1号,其余4个品种为中等抗旱。该抗旱性评价结果与这些品种在生产上的表现一致,干旱胁迫2小时花生品种叶片AhNCED1基因的表达量变化与其抗旱性一致。因此,AhNCED1基因表达量可作为苗期花生品种抗旱性等级的量化指标之一。
The comprehensive membership function method was used to evaluate the drought resistance of eight peanut seedlings in different habitats. At the same time, the change of AhNCED1 gene expression in leaves of various peanut varieties was analyzed at 2 hours and 12 hours after simulated drought stress to explore the relationship between drought resistance and leaf AhNCED1 gene expression (Arachis hypogaea) to determine the drought resistance level of peanut through the expression of AhNCED1 gene during seedling stage. The results showed that the expression of AhNCED1 gene in leaves of all varieties increased rapidly at 2 hours after drought treatment, and the difference was significant compared with that before treatment. The expression of AhNCED1 gene in peanut leaves at 12 hours of drought treatment decreased but still higher than that before treatment Level. The comprehensive membership function value quantitatively reflects the order of drought resistance of all varieties: Huayu 24> Fuhua 13> Yueyou 7> Zhonghua 15> Zhonghua 16> Hanghua 2> Fuhua 9> Beihai 1. The change of AhNCED1 expression in peanut leaves at 2 hours of drought treatment was in the order of Fuhua 13> Huayu 24> Zhonghua 16> Zhonghua 15> Hanghua 2> Yueyou 7> Beihai 1> Fukuda 9. Among them, the drought-resistant varieties were Huayu 24 and Fuhua 13, and the cultivars with weak drought resistance were Fuhua 9 and Beihai 1, and the remaining 4 cultivars were moderately drought-resistant. The results of drought resistance evaluation showed that the expression of AhNCED1 gene in leaves of peanut varieties at 2 hours drought stress was in accordance with the drought resistance. Therefore, the amount of AhNCED1 gene expression can be used as one of the quantitative indicators of the drought resistance level of seedling peanut varieties.