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槐糖脂是酵母菌产生的一种重要的生物表面活性剂,具有抗肿瘤活性.纳米羟基磷灰石(Nano-hydroxyapatite,n HA)因具有良好的生物活性及生物相容性而被广泛应用于临床载体,与药物相负载.通过n HA与内酯型双乙酰槐糖脂(Lactonic diacetyl-sophorolipids,L-SLs)最适搭载率的确定及负载后药物缓释能力的测试、细胞毒性研究、细胞形态观察,探讨搭载后的L-SLs对人宫颈癌Hela细胞的抑制作用.结果显示:最适搭载率为1:5,且L-SLs浓度为5.0 mg/m L时负载率可以达到最大值99.47%,负载后药物缓释时间为14 h,L-SLs对人宫颈癌Hela细胞的增殖有良好的抑制作用,并且细胞形态发生了明显的变化,细胞膜鼓出,体积减小,细胞肿胀裂解成碎片、细胞解体.本研究表明,L-SLs能够抑制人宫颈癌Hela细胞的增殖,n HA搭载L-SLs后能够延长槐糖脂的释放时间.
Sophorolipids are an important biosurfactant produced by yeast and have antitumor activity.Nano-hydroxyapatite (n HA) has been widely used because of its good bioactivity and biocompatibility In clinical carriers and drug-loaded phase.According to the determination of the optimum loading ratio of n HA and Lactonic diacetyl-sophorolipids (L-SLs) and the test of drug sustained-release ability after loading, the cytotoxicity , The morphology of the cells was observed to investigate the inhibitory effect of L-SLs loaded on human cervical cancer Hela cells.The results showed that the optimal loading rate was 1: 5 and the loading rate of L-SLs was 5.0 mg / m L L-SLs could inhibit the proliferation of human cervical cancer Hela cells in a dose-dependent manner with the maximum value of 99.47% and the sustained release time of drug was 14 h. The morphology of cells was obviously changed, the cell membrane was bulged, the volume decreased, The results showed that L-SLs can inhibit the proliferation of human cervical cancer Hela cells, n-HA-loaded L-SLs can prolong the release time of sophorolipids.