目的了解分析广东省各级结核病网络实验室能力现状,为下一步开展全省结核病实验室网络建设提供决策依据。方法向省级、地市级和县级结核病实验室发放实验室信息调查表,内容包括实验室设备、人力资源、开展项目和质量、科研工作等,回收后对调查表进行审核和统计分析。结果省级结核病实验室承担全省参比实验室工作及具备较强科研实力,地市级实验室主要开展结核分枝杆菌分离培养(81.0%)、药敏试验(81.0%)和分子生物学检查(47.6%),县(区)级实验室主要开展痰涂片(100%)和结核分枝杆菌分离培养(40.4%)。人力资源方面,全省各级结核病实验室共有工作人员407人,其中大专及以上学历占70.5%,初级和中级职称分别为76.9%、18.4%,医学检验专业占90.9%。目前全省结核病实验室均配备了Ⅱ级生物安全柜,90%的实验室装备了恒温培养箱、漩涡振荡器等开展培养和药敏试验必需的设备。结论应继续加大对结核病实验室资金投入和技术培训力度,进一步加强实验室能力建设,使其尽快达到“十二五”结核病防治规划要求。 Objective To understand and analyze the status quo of TB laboratories in Guangdong Province at all levels and provide decision-making basis for the construction of TB laboratories in the province in the next step. Methods The laboratory information questionnaire was distributed to provincial, municipal, and county TB laboratories, including laboratory equipment, human resources, projects and quality, and scientific research work. The questionnaires were reviewed and statistically analyzed after recovery. Results The provincial TB laboratory undertakes the work of reference laboratories in the province and possesses strong scientific research strength. The prefecture-level laboratories mainly carry out isolation and culture of Mycobacterium tuberculosis (81.0%), drug sensitivity test (81.0%) and molecular biology Examination (47.6%), county (district) level laboratory mainly smear (100%) and Mycobacterium tuberculosis isolation culture (40.4%). In terms of human resources, there are 407 staff members in TB laboratories at all levels in the province, of whom 70.5% have college degree or above, 76.9% have junior and intermediate professional titles, and 18.4% have intermediate professional titles, accounting for 90.9% of medical examinations. At present, all the TB laboratories in the province are equipped with Class II biological safety cabinets, and 90% of laboratories are equipped with equipment necessary for culturing and susceptibility testing such as incubators and vortex oscillators. Conclusion It is necessary to continue to increase funding for TB laboratories and provide technical training so as to further strengthen laboratory capacity building so that it can meet the requirements of TB prevention and control planning as soon as possible.