目的　研究苯对卵母细胞和 1细胞合子雌原核染色体非整倍体率的影响。方法　成年雌性NIH小鼠 ,1次灌胃 (942、1881和 376 2mg/kg)与多次吸入 (70 6、192 2和 486 4mg/m3 )染毒 ,灌胃后雌雄鼠以 1∶1同笼过夜 ,收集卵母细胞和 1细胞合子作细胞遗传学分析 ,测定非整倍体率。结果　小鼠吸入染毒 3个剂量组的第 2次减数分裂中期 (MⅡ )卵母细胞非整倍体率分别为 7 0 6 %、7 5 0 %和9 76 % ,明显高于对照组 (1 30 % ) ,有剂量 效应关系 ,同时也观察到第 1次减数分裂中期 (MⅠ )卵母细胞减数分裂停滞 ,MⅠ卵母细胞频率分别为 1 16 %、3 6 1%和 5 75 % ,高于对照组 (0 0 0 % ) ,有剂量 效应关系 ,在灌胃组仅见高剂量组MⅡ卵母细胞非整倍体率增高 ,1细胞合子雌原核非整倍体率未见增高。结论　吸入或灌胃给小鼠高剂量苯 ,可诱导MⅡ卵母细胞非整倍体率增高 Objective To study the effect of benzene on the aneuploidy rate of zygotes in oocyte and zygote of zygotes. Methods Adult female NIH mice were inoculated intragastrically (942, 1881 and 376 2mg / kg) and multiple inhalation (70, 6, 192 and 486 mg / m3) Cages were overnight, oocytes and zygotes were collected for cytogenetic analysis and aneuploidy rates were determined. Results The rates of aneuploidy in the second metaphase of the second stage of meiosis (M Ⅱ) were 70.6%, 75.0% and 976%, respectively, which were significantly higher than those of the control group (1 30%), and there was a dose-response relationship. The first meiotic metaphase (MI) oocyte meiosis stagnation was observed. The frequencies of MⅠ oocyte were 16%, 361% and 5% 75%, higher than the control group (0%), dose-response relationship, in the intragastric administration group only high dose of M Ⅱ oocyte aneuploidy rate increased, 1 cell zygotes progesterone in female aneuploidy rate was not seen Increase. Conclusion Inhalation or intragastric administration of high dose benzene to mice can induce MⅡ oocyte aneuploidy rate increase